Preparation and Properties of 5'-Nucleotidase from Smooth Muscle of Small Intestine

Preparation and Properties of 5'-Nucleotidase from Smooth Muscle of Small Intestine

5'-Nucleotidase prepared from smooth muscle is strongly inhibited by ADP. This inhibition is much stronger than the previously described inhibition of 5'-nucleotidase by ATP. The α,β-methylenephosphonate analogue of ADP is an exceptionally powerful inhibitor of the enzyme. The enzyme pos...

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Veröffentlicht in:The Journal of biological chemistry 1970-12, Vol.245 (23), p.6274-6280
Hauptverfasser: Burger, R M, Lowenstein, J M
Format: Artikel
Sprache:eng
Schlagworte:
Adenine Nucleotides - pharmacology
Animals
Buffers
Chemical Phenomena
Chemistry
Hydrogen-Ion Concentration
Intestine, Small - enzymology
Magnesium - pharmacology
Muscle, Smooth - enzymology
Nitrophenols
Nucleotidases - antagonists & inhibitors
Phosphoric Monoester Hydrolases - metabolism
Phosphotransferases - metabolism
Swine
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Zusammenfassung:5'-Nucleotidase prepared from smooth muscle is strongly inhibited by ADP. This inhibition is much stronger than the previously described inhibition of 5'-nucleotidase by ATP. The α,β-methylenephosphonate analogue of ADP is an exceptionally powerful inhibitor of the enzyme. The enzyme possesses three pH optima. The lowest of these optima is observed only when the enzyme is not inhibited by buffer anions; the highest is observed only in the presence of magnesium ions.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)62605-5