Platelets prevent acute hepatitis induced by anti-fas antibody

Background and Aim: Platelets provide many functions in the body, especially to the liver. The purpose of this study is to investigate the effect of thrombocytosis with acute hepatitis induced by anti‐Fas antibody and its mechanism. Methods: Acute hepatitis was induced by administration of anti‐Fas antibody in normal and thrombocytotic C57BL6J mice. For thrombocytosis, thrombopoietin; PEG‐rHuMGDF was injected 5 days before and just prior to administration of anti‐Fas antibody. To investigate the mechanisms, hepatocyte cell line (AML12) and sinusoidal endothelial cell line (M1) were induced apoptosis by staurosporine. They were cultured with platelets or thrombopoietin. Examination items were as follows: platelet number, alanine aminotransferase (ALT), histological findings, TUNEL (TdT‐mediated dUTP‐biotin Nick End Labeling) staining, and the expression of proteins associated with apoptosis in vivo and in vitro. Results: Platelets were significantly increased in the thrombocytotic group (P