Studies on the initiation of protein synthesis in animal tissues

Studies on the initiation of protein synthesis in animal tissues

Two methionine accepting tRNAs have been separated from rabbit liver tRNA by column chromatography on BD-cellulose. One of the species (Met-tRNA F Met) can be formylated using an Escherichia coli transformylase preparation, and maximal binding of this species to brain ribosomes is achieved in the pr...

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Veröffentlicht in:Biochemical and biophysical research communications 1970-01, Vol.41 (1), p.78-84
Hauptverfasser: Kerwar, S.S., Spears, Carlos, Weissbach, Herbert
Format: Artikel
Sprache:eng
Schlagworte:
Acyltransferases
Ammonium Chloride
Animals
Brain - cytology
Cattle
Chromatography, Ion Exchange
Escherichia coli - enzymology
Formates
Guanine Nucleotides
Kinetics
Liver - metabolism
Methionine
Protein Biosynthesis
Rabbits
Ribosomes - metabolism
RNA, Transfer - isolation & purification
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Beschreibung
Zusammenfassung:Two methionine accepting tRNAs have been separated from rabbit liver tRNA by column chromatography on BD-cellulose. One of the species (Met-tRNA F Met) can be formylated using an Escherichia coli transformylase preparation, and maximal binding of this species to brain ribosomes is achieved in the presence of AUG, GTP and a 0.5 M NH 4Cl extract of the ribosomes. Transferase I cannot replace the NH 4Cl extract in the binding assay. The other tRNA species (Met-tRNA Met) requires GTP and transferase I for binding to ribosomes.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(70)90471-7