The osteoinductive effect of chitosan-collagen composites around pure titanium implant surfaces in rats

Kung S, Devlin H, Fu E, Ho K‐Y, Liang S‐Y, Hsieh Y‐D. The osteoinductive effect of chitosan–collagen composites around pure titanium implant surfaces in rats. J Periodont Res 2011; 46: 126–133. © 2010 John Wiley & Sons A/S Background and Objective: The enhancing effects of chitosan on activation of platelets and differentiation of osteoprogenitor cells have been demonstrated in vitro. The purpose of this study was to evaluate the in vivo osteoinductive effect of chitosan–collagen composites around pure titanium implant surfaces. Material and Methods: Chitosan–collagen composites containing chitosan of different molecular weights (450 and 750 kDa) were wrapped onto titanium implants and embedded into the subcutaneous area on the back of 15 Sprague–Dawley rats. The control consisted of implants wrapped with plain collagen type I membranes. Implants and surrounding tissues were retrieved 6 wks after surgery and identified by Alizarin red and Alcian blue whole mount staining. The newly formed structures in the test groups were further analyzed by Toluidine blue and Masson–Goldner trichrome staining, and immunohistochemical staining with osteopontin and alkaline phosphotase. The bone formation parameters of the new bone in the two test groups were measured and compared. Results: New bone formed ectopically in both chitosan–collagen groups, whereas no bone induction occurred in the negative control group. These newly formed bone‐like structures were further confirmed by immunohistochemical staining. Comparison of bone parameters of the newly induced bone revealed no statistically significant differences between the 450 and 750 kDa chitosan–collagen groups. Conclusion: Our results demonstrated that chitosan–collagen composites might induce in vivo new bone formation around pure titanium implant surfaces. Different molecular weights of chitosan did not show significantly different effects on the osteoinductive potential of the test materials.