Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coli

The formation of pulse labelled DNA which remains firmly attached to membrane after further growth in unlabelled medium has been studied at different periods of the cell cycle using synchronized cultures of Escherichia coli. The maximal labelling of this species of membrane bound DNA occurs near the...

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Veröffentlicht in:Biochemical and biophysical research communications 1970-10, Vol.41 (1), p.157-162
Hauptverfasser: Fielding, Phoebe, Fred Fox, C.
Format: Artikel
Sprache:eng
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Zusammenfassung:The formation of pulse labelled DNA which remains firmly attached to membrane after further growth in unlabelled medium has been studied at different periods of the cell cycle using synchronized cultures of Escherichia coli. The maximal labelling of this species of membrane bound DNA occurs near the time of cell division. This provides evidence for association of DNA at the replication origin with the cell membrane.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(70)90482-1