A recording potentiometric/spectrophotometric titrator

A recording potentiometric/spectrophotometric titrator

A system for the simultaneous recording of spectrophotometric and potentiometric titrations at constant temperature is described. Application to the determination of the p K′ of the ionization of the iron-bound water molecule in horse and in sperm whale ferrimyoglobin and to the study of the attachm...

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Veröffentlicht in:Analytical biochemistry 1967-12, Vol.21 (3), p.401-410
1. Verfasser: O'Hagan, John E.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Binding Sites
Cetacea
Hemoglobins
Horses
Hydrogen-Ion Concentration
Iron
Myoglobin
Nickel
Porphyrins
Potentiometry - instrumentation
Protein Binding
Spectrophotometry - instrumentation
Water
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Beschreibung
Zusammenfassung:A system for the simultaneous recording of spectrophotometric and potentiometric titrations at constant temperature is described. Application to the determination of the p K′ of the ionization of the iron-bound water molecule in horse and in sperm whale ferrimyoglobin and to the study of the attachment of nickel mesoporphyrin to human apohemoglobin A is given.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(67)90314-4