Inhibition of Lipolytic Activity by Lipemic Plasma Induced by Oral Administration of Fat

1 The inhibition of post-heparin lipolytic activity was investigated by rate measurement of liberated free fatty acids with zero order kinetics in the medium added with lipemic plasma obtained after fat load. Platelet-poor fasting, platelet-rich lipemic as well as platelet-poor lipemic plasma obtained from students revealed the inhibitory action on lipolytic activity of post-heparin plasma in vitro, but the most remarkable inhibition was noted when platelet-poor lipemic plasma was added. 2 Three hours after the oral administration of olive oil in rats, a striking increase was noticed in hepatic and plasma triglyceride compared with those of glucose-treated rats. 3 The lipemic plasma obtained from oil-treated rats showed the stronger inhibitory activity on the post-heparin lipolytic activity in vitro in comparison with the normal plasma from glucose-treated rats.