Phospholipids of red cells and blood plasma in adults, newborn infants, and patients with Rh erythroblastosis
The concentration of total and individual phospholipids was determined in erythrocytes and plasma of adults, neonates, and newborn infants with Rh erythroblastosis. After extraction the phospholipids were separated by one-dimensional, thin layer chromatography into six fractions: phosphatidylethanol...
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| Veröffentlicht in: | Pediatrics (Evanston) 1970-08, Vol.46 (2), p.259-266 |
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| creator | Hürter, P Schröter, W Schedel, I Gercken, G |
| description | The concentration of total and individual phospholipids was determined in erythrocytes and plasma of adults, neonates, and newborn infants with Rh erythroblastosis. After extraction the phospholipids were separated by one-dimensional, thin layer chromatography into six fractions: phosphatidylethanolamine, phosphatidylserine, phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, and nonidentified phospholipids. In normal neonates the total amount of red cell and plasma phospholipids was significantly decreased (1.32±0.20 µmoles/ml plasma, p |
| doi_str_mv | 10.1542/peds.46.2.259 |
| format | Article |
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After extraction the phospholipids were separated by one-dimensional, thin layer chromatography into six fractions: phosphatidylethanolamine, phosphatidylserine, phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, and nonidentified phospholipids. In normal neonates the total amount of red cell and plasma phospholipids was significantly decreased (1.32±0.20 µmoles/ml plasma, p<0.001; 3.03±0.65x10-10 µmoles/erythrocyte, p<0.001) when compared with the data of the adults (2.88±0.69µmoles/ml plasma; 4.11± 0.37x10-10 µmoles/erythrocyte). All the individual phospholipids were diminished in the plasma. In erythrocytes only the amount of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine was reduced, whereas the sphingomyelin content was unchanged, i.e., it was increased when expressed as percent of total phospholipids (adult blood erythrocytes 24.2±0.8%; cord blood erythrocytes 29.7±2.8%, p<0.001). Thus, the sphingomyehn/phosphatidylcholine ratio was markedly higher in cord blood erythrocytes (1.03) than in adult red cells (0.81). In newborn infants with Rh erythroblastosis, the decrease of total and individual red cell phospholipids was less striking than in normal neonates. In the plasma the phospholipid patterns of the two neonate groups were identical. It is suggested that the total and individual phospholipid alterations in fetal red cells might be due to the low plasma levels of phospholipids in neonates.</description><identifier>ISSN: 0031-4005</identifier><identifier>EISSN: 1098-4275</identifier><identifier>DOI: 10.1542/peds.46.2.259</identifier><identifier>PMID: 5464557</identifier><language>eng</language><publisher>United States</publisher><subject>Adult ; Blood ; Chromatography, Thin Layer ; Erythroblastosis, Fetal - blood ; Erythrocytes - analysis ; Female ; Humans ; Infant, Newborn ; Methods ; Phosphates - blood ; Phosphatidylcholines - blood ; Phosphatidylethanolamines - blood ; Phospholipids - blood ; Plasma - analysis ; Pregnancy ; Sphingomyelins - blood ; Umbilical Cord</subject><ispartof>Pediatrics (Evanston), 1970-08, Vol.46 (2), p.259-266</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c288t-bb0d5d3800cb15d83baf0270263bc755b84bb116cf36d80d641165b699c28de93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5464557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hürter, P</creatorcontrib><creatorcontrib>Schröter, W</creatorcontrib><creatorcontrib>Schedel, I</creatorcontrib><creatorcontrib>Gercken, G</creatorcontrib><title>Phospholipids of red cells and blood plasma in adults, newborn infants, and patients with Rh erythroblastosis</title><title>Pediatrics (Evanston)</title><addtitle>Pediatrics</addtitle><description>The concentration of total and individual phospholipids was determined in erythrocytes and plasma of adults, neonates, and newborn infants with Rh erythroblastosis. After extraction the phospholipids were separated by one-dimensional, thin layer chromatography into six fractions: phosphatidylethanolamine, phosphatidylserine, phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, and nonidentified phospholipids. In normal neonates the total amount of red cell and plasma phospholipids was significantly decreased (1.32±0.20 µmoles/ml plasma, p<0.001; 3.03±0.65x10-10 µmoles/erythrocyte, p<0.001) when compared with the data of the adults (2.88±0.69µmoles/ml plasma; 4.11± 0.37x10-10 µmoles/erythrocyte). All the individual phospholipids were diminished in the plasma. In erythrocytes only the amount of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine was reduced, whereas the sphingomyelin content was unchanged, i.e., it was increased when expressed as percent of total phospholipids (adult blood erythrocytes 24.2±0.8%; cord blood erythrocytes 29.7±2.8%, p<0.001). Thus, the sphingomyehn/phosphatidylcholine ratio was markedly higher in cord blood erythrocytes (1.03) than in adult red cells (0.81). In newborn infants with Rh erythroblastosis, the decrease of total and individual red cell phospholipids was less striking than in normal neonates. In the plasma the phospholipid patterns of the two neonate groups were identical. It is suggested that the total and individual phospholipid alterations in fetal red cells might be due to the low plasma levels of phospholipids in neonates.</description><subject>Adult</subject><subject>Blood</subject><subject>Chromatography, Thin Layer</subject><subject>Erythroblastosis, Fetal - blood</subject><subject>Erythrocytes - analysis</subject><subject>Female</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Methods</subject><subject>Phosphates - blood</subject><subject>Phosphatidylcholines - blood</subject><subject>Phosphatidylethanolamines - blood</subject><subject>Phospholipids - blood</subject><subject>Plasma - analysis</subject><subject>Pregnancy</subject><subject>Sphingomyelins - blood</subject><subject>Umbilical Cord</subject><issn>0031-4005</issn><issn>1098-4275</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1LxDAUxIMo67p69Cjk5MnW13w1PYr4BQuK6DkkTUorbVOTlmX_e1t28fSY4TcDbxC6ziDNOCP3g7MxZSIlKeHFCVpnUMiEkZyfojUAzRIGwM_RRYw_AMB4TlZoxZlgnOdr1H3UPg61b5uhsRH7CgdncenaNmLdW2xa7y0eWh07jZseazu1Y7zDvdsZH_rZqnS_GAs86LFxs8K7ZqzxZ41d2I918GaOjz428RKdVbqN7up4N-j7-enr8TXZvr-8PT5sk5JIOSbGgOWWSoDSZNxKanQFJAciqClzzo1kxmSZKCsqrAQr2Cy4EUUx560r6AbdHnqH4H8nF0fVNXF5SvfOT1FJJjgFSWcwOYBl8DEGV6khNJ0Oe5WBWuZVy7yKCUXUPO_M3xyLJ9M5-08f96R_zUh3wg</recordid><startdate>197008</startdate><enddate>197008</enddate><creator>Hürter, P</creator><creator>Schröter, W</creator><creator>Schedel, I</creator><creator>Gercken, G</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197008</creationdate><title>Phospholipids of red cells and blood plasma in adults, newborn infants, and patients with Rh erythroblastosis</title><author>Hürter, P ; Schröter, W ; Schedel, I ; Gercken, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c288t-bb0d5d3800cb15d83baf0270263bc755b84bb116cf36d80d641165b699c28de93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Adult</topic><topic>Blood</topic><topic>Chromatography, Thin Layer</topic><topic>Erythroblastosis, Fetal - blood</topic><topic>Erythrocytes - analysis</topic><topic>Female</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Methods</topic><topic>Phosphates - blood</topic><topic>Phosphatidylcholines - blood</topic><topic>Phosphatidylethanolamines - blood</topic><topic>Phospholipids - blood</topic><topic>Plasma - analysis</topic><topic>Pregnancy</topic><topic>Sphingomyelins - blood</topic><topic>Umbilical Cord</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hürter, P</creatorcontrib><creatorcontrib>Schröter, W</creatorcontrib><creatorcontrib>Schedel, I</creatorcontrib><creatorcontrib>Gercken, G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pediatrics (Evanston)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hürter, P</au><au>Schröter, W</au><au>Schedel, I</au><au>Gercken, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phospholipids of red cells and blood plasma in adults, newborn infants, and patients with Rh erythroblastosis</atitle><jtitle>Pediatrics (Evanston)</jtitle><addtitle>Pediatrics</addtitle><date>1970-08</date><risdate>1970</risdate><volume>46</volume><issue>2</issue><spage>259</spage><epage>266</epage><pages>259-266</pages><issn>0031-4005</issn><eissn>1098-4275</eissn><abstract>The concentration of total and individual phospholipids was determined in erythrocytes and plasma of adults, neonates, and newborn infants with Rh erythroblastosis. After extraction the phospholipids were separated by one-dimensional, thin layer chromatography into six fractions: phosphatidylethanolamine, phosphatidylserine, phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, and nonidentified phospholipids. In normal neonates the total amount of red cell and plasma phospholipids was significantly decreased (1.32±0.20 µmoles/ml plasma, p<0.001; 3.03±0.65x10-10 µmoles/erythrocyte, p<0.001) when compared with the data of the adults (2.88±0.69µmoles/ml plasma; 4.11± 0.37x10-10 µmoles/erythrocyte). All the individual phospholipids were diminished in the plasma. In erythrocytes only the amount of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine was reduced, whereas the sphingomyelin content was unchanged, i.e., it was increased when expressed as percent of total phospholipids (adult blood erythrocytes 24.2±0.8%; cord blood erythrocytes 29.7±2.8%, p<0.001). Thus, the sphingomyehn/phosphatidylcholine ratio was markedly higher in cord blood erythrocytes (1.03) than in adult red cells (0.81). In newborn infants with Rh erythroblastosis, the decrease of total and individual red cell phospholipids was less striking than in normal neonates. In the plasma the phospholipid patterns of the two neonate groups were identical. It is suggested that the total and individual phospholipid alterations in fetal red cells might be due to the low plasma levels of phospholipids in neonates.</abstract><cop>United States</cop><pmid>5464557</pmid><doi>10.1542/peds.46.2.259</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adult Blood Chromatography, Thin Layer Erythroblastosis, Fetal - blood Erythrocytes - analysis Female Humans Infant, Newborn Methods Phosphates - blood Phosphatidylcholines - blood Phosphatidylethanolamines - blood Phospholipids - blood Plasma - analysis Pregnancy Sphingomyelins - blood Umbilical Cord |
| title | Phospholipids of red cells and blood plasma in adults, newborn infants, and patients with Rh erythroblastosis |
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