Adaptation of multiple internal reflection (MIR) spectroscopy to the identification of steroids from thin-layer chromatographic zones

Multiple internal reflection (MIR) spectroscopy has been used to identify microgram quantities of steroids obtained from the eluates of thin-layer chromatography (TLC) zones without the need for any further purification. The use of these combined techniques (MIR and TLC) becomes a powerful tool for the identification of compounds from biological materials. This technique has permitted us to study structural changes that may occur under varying conditions. It has also been used to follow different stages of a methodology being developed as well as to verify the chemical principles behind methodology already in use.