PERCHLORIC ACID EXTRACTION OF DEOXYRIBONUCLEIC ACID FROM THIN SECTIONS OF EPON-ARALDITE-EMBEDDED MATERIAL

PERCHLORIC ACID EXTRACTION OF DEOXYRIBONUCLEIC ACID FROM THIN SECTIONS OF EPON-ARALDITE-EMBEDDED MATERIAL

A method is presented for the extraction of deoxyribonucleic acid from thin sections of tissue fixed in glutaraldehyde and osmium tetroxide and embedded in water-insoluble epoxy resin. Incubation of thin sections in 20% perchloric acid at 60°C for 8 hr produced electron-transparent bands in the poly...

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Veröffentlicht in:The journal of histochemistry and cytochemistry 1970-07, Vol.18 (7), p.510-514
1. Verfasser: DOUGLAS, WILLIAM H. J
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Chromosomes - analysis
Deoxyribonucleases - pharmacology
DNA - isolation & purification
Drosophila
Electron Transport
Histological Techniques
Microscopy, Electron
Perchlorates - pharmacology
Salivary Glands - analysis
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container_title The journal of histochemistry and cytochemistry
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creator DOUGLAS, WILLIAM H. J
description A method is presented for the extraction of deoxyribonucleic acid from thin sections of tissue fixed in glutaraldehyde and osmium tetroxide and embedded in water-insoluble epoxy resin. Incubation of thin sections in 20% perchloric acid at 60°C for 8 hr produced electron-transparent bands in the polytene chromosomes from Drosophila salivary glands. Chromosome bands were also rendered electron-transparent when hydroxypropylmethacrylate-embedded thin sections were digested with deoxyribonuclease or extracted with perchloric acid. Nucleolar ribonucleic acid was unaffected by the perchloric acid extraction.
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subjects Animals
Chromosomes - analysis
Deoxyribonucleases - pharmacology
DNA - isolation & purification
Drosophila
Electron Transport
Histological Techniques
Microscopy, Electron
Perchlorates - pharmacology
Salivary Glands - analysis
title PERCHLORIC ACID EXTRACTION OF DEOXYRIBONUCLEIC ACID FROM THIN SECTIONS OF EPON-ARALDITE-EMBEDDED MATERIAL
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