PERCHLORIC ACID EXTRACTION OF DEOXYRIBONUCLEIC ACID FROM THIN SECTIONS OF EPON-ARALDITE-EMBEDDED MATERIAL

A method is presented for the extraction of deoxyribonucleic acid from thin sections of tissue fixed in glutaraldehyde and osmium tetroxide and embedded in water-insoluble epoxy resin. Incubation of thin sections in 20% perchloric acid at 60°C for 8 hr produced electron-transparent bands in the poly...

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Veröffentlicht in:	The journal of histochemistry and cytochemistry 1970-07, Vol.18 (7), p.510-514
1. Verfasser:	DOUGLAS, WILLIAM H. J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromosomes - analysis Deoxyribonucleases - pharmacology DNA - isolation & purification Drosophila Electron Transport Histological Techniques Microscopy, Electron Perchlorates - pharmacology Salivary Glands - analysis
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Zusammenfassung:	A method is presented for the extraction of deoxyribonucleic acid from thin sections of tissue fixed in glutaraldehyde and osmium tetroxide and embedded in water-insoluble epoxy resin. Incubation of thin sections in 20% perchloric acid at 60°C for 8 hr produced electron-transparent bands in the polytene chromosomes from Drosophila salivary glands. Chromosome bands were also rendered electron-transparent when hydroxypropylmethacrylate-embedded thin sections were digested with deoxyribonuclease or extracted with perchloric acid. Nucleolar ribonucleic acid was unaffected by the perchloric acid extraction.
ISSN:	0022-1554 1551-5044
DOI:	10.1177/18.7.510