Ribonuclease B of bovine milk

Ribonuclease B of bovine milk

Ribonuclease B has been isolated from 757 liters of milk; pilot plant facilities were used to provide a crude fraction. Final purification was achieved by gel filtration on Sephadex G-75 and chromatography on IRC-50. Milk ribonuclease A was also isolated by this procedure. Milk ribonuclease B has an...

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Veröffentlicht in:Archives of biochemistry and biophysics 1967-08, Vol.121 (2), p.317-324
Hauptverfasser: Bingham, Elizabeth W., Kalan, Edwin B.
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acids - analysis
Animals
Cattle
Chromatography
Chromatography, Gel
Chromatography, Paper
Electrophoresis
Female
Glycoproteins - analysis
Hexosamines - analysis
milk
Milk - analysis
Pancreas - enzymology
purification
ribonucleases
Ribonucleases - analysis
Spectrophotometry
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Zusammenfassung:Ribonuclease B has been isolated from 757 liters of milk; pilot plant facilities were used to provide a crude fraction. Final purification was achieved by gel filtration on Sephadex G-75 and chromatography on IRC-50. Milk ribonuclease A was also isolated by this procedure. Milk ribonuclease B has an amino acid composition which is identical to pancreatic ribonuclease A, milk ribonuclease A, and pancreatic ribonuclease B. It is a glycoprotein containing 4.2% hexosamine (3.0% glucosamine plus 1.2% galactosamine) and 5.17% mannose. It differs from pancreatic ribonuclease B, which contains 2.16% glucosamine, no galactosamine, and 5.7% mannose.
ISSN:0003-9861
1096-0384
DOI:10.1016/0003-9861(67)90082-3