Detection and differentiation of sugars on paper by the p-anisidine/periodate reaction

Detection and differentiation of sugars on paper by the p-anisidine/periodate reaction

A two-reagent system, p-anisidine/ethanol followed by sodium periodate/acetone solution, is described for the detection and differentiation of sugars and sugar derivatives by their color reactions. The sensitivity of the method ranges from 1 to 20 μg, depending on the substances assayed. Polyols, su...

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Veröffentlicht in:Analytical biochemistry 1967-09, Vol.20 (3), p.419-422
Hauptverfasser: Veiga, L.A., Chandelier, E.L.
Format: Artikel
Sprache:eng
Schlagworte:
Acetone
Amino Sugars - analysis
Aniline Compounds
Carbohydrates - analysis
Chemical Phenomena
Chemistry
Chromatography, Paper
Ethanol
Hexoses - analysis
Pentoses - analysis
Periodic Acid
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Zusammenfassung:A two-reagent system, p-anisidine/ethanol followed by sodium periodate/acetone solution, is described for the detection and differentiation of sugars and sugar derivatives by their color reactions. The sensitivity of the method ranges from 1 to 20 μg, depending on the substances assayed. Polyols, sugar acids, 2-deoxy sugars, amino sugars and their acetyl derivatives, pentoses, and hexoses can be identified and characterized by this method.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(67)90286-2