Isolation and characterization of histones from Ehrlich ascites tumor after in vitro labeling with lysine- 14C
After in vitro incubation of a nuclear preparation from Ehrlich ascites tumor with lysine- 14C under optimal conditions, labeled histones were isolated from the DNA-histone complex. By a combination of extraction and chromatographic procedures 15 different fractions were isolated. These fractions sh...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1967-03, Vol.118 (3), p.631-636 |
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| container_end_page | 636 |
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| container_issue | 3 |
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| container_title | Archives of biochemistry and biophysics |
| container_volume | 118 |
| creator | Ohly, Karl W. Mehta, Narendra G. Mourkides, George A. Alivisatos, Spyridon G.A. |
| description | After
in vitro incubation of a nuclear preparation from Ehrlich ascites tumor with lysine-
14C under optimal conditions, labeled histones were isolated from the DNA-histone complex. By a combination of extraction and chromatographic procedures 15 different fractions were isolated. These fractions showed specific radioactivities ranging between 100 and 500 dpm per micromole of lysine. Starch-gel electrophoretic separation of each fraction showed that they were all mixtures composed of several components. |
| doi_str_mv | 10.1016/0003-9861(67)90398-0 |
| format | Article |
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in vitro incubation of a nuclear preparation from Ehrlich ascites tumor with lysine-
14C under optimal conditions, labeled histones were isolated from the DNA-histone complex. By a combination of extraction and chromatographic procedures 15 different fractions were isolated. These fractions showed specific radioactivities ranging between 100 and 500 dpm per micromole of lysine. Starch-gel electrophoretic separation of each fraction showed that they were all mixtures composed of several components.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(67)90398-0</identifier><identifier>PMID: 6048214</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acids - analysis ; Animals ; Carbon Isotopes ; Carcinoma, Ehrlich Tumor - metabolism ; Chromatography, Gel ; DNA, Neoplasm ; Histones - analysis ; Lysine - metabolism ; Male ; Mice ; Neoplasm Proteins - analysis</subject><ispartof>Archives of biochemistry and biophysics, 1967-03, Vol.118 (3), p.631-636</ispartof><rights>1967</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c289t-b2757450a16f1657fc53087f76936d81d6fbdfde8310e76efd14310bb397ca253</citedby><cites>FETCH-LOGICAL-c289t-b2757450a16f1657fc53087f76936d81d6fbdfde8310e76efd14310bb397ca253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-9861(67)90398-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6048214$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohly, Karl W.</creatorcontrib><creatorcontrib>Mehta, Narendra G.</creatorcontrib><creatorcontrib>Mourkides, George A.</creatorcontrib><creatorcontrib>Alivisatos, Spyridon G.A.</creatorcontrib><title>Isolation and characterization of histones from Ehrlich ascites tumor after in vitro labeling with lysine- 14C</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>After
in vitro incubation of a nuclear preparation from Ehrlich ascites tumor with lysine-
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in vitro incubation of a nuclear preparation from Ehrlich ascites tumor with lysine-
14C under optimal conditions, labeled histones were isolated from the DNA-histone complex. By a combination of extraction and chromatographic procedures 15 different fractions were isolated. These fractions showed specific radioactivities ranging between 100 and 500 dpm per micromole of lysine. Starch-gel electrophoretic separation of each fraction showed that they were all mixtures composed of several components.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6048214</pmid><doi>10.1016/0003-9861(67)90398-0</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9861 |
| ispartof | Archives of biochemistry and biophysics, 1967-03, Vol.118 (3), p.631-636 |
| issn | 0003-9861 1096-0384 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84543863 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amino Acids - analysis Animals Carbon Isotopes Carcinoma, Ehrlich Tumor - metabolism Chromatography, Gel DNA, Neoplasm Histones - analysis Lysine - metabolism Male Mice Neoplasm Proteins - analysis |
| title | Isolation and characterization of histones from Ehrlich ascites tumor after in vitro labeling with lysine- 14C |
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