Chromatography of nucleic acid components on cyclodextrin gels

Chromatography of nucleic acid components on cyclodextrin gels

The application of gels consisting of cross-linked cycloheptaamylose to column chromatographic separations of nucleic acid derivatives has been described. Separations of various nucleotide and nucleoside mixtures were achieved, most notably those containing adenine. Separation is believed to rely on...

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Veröffentlicht in:Analytical biochemistry 1970-02, Vol.33 (2), p.209-217
1. Verfasser: Hoffman, Jerald L.
Format: Artikel
Sprache:eng
Schlagworte:
Adenine Nucleotides - analysis
Chromatography, Gel
DNA - analysis
Gels
Methods
Nucleic Acids - analysis
Nucleotides - analysis
Oligosaccharides
RNA - analysis
Spectrophotometry
Temperature
Ultraviolet Rays
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Zusammenfassung:The application of gels consisting of cross-linked cycloheptaamylose to column chromatographic separations of nucleic acid derivatives has been described. Separations of various nucleotide and nucleoside mixtures were achieved, most notably those containing adenine. Separation is believed to rely on principles of inclusion complex formation similar to those for cyclodextrin molecules in solution.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(70)90288-5