Colcemid Sensitivity of Fission Yeast and the Isolation of Colcemid-Resistant Mutants

Cell division of the fission yeast, Schizosaccharomyces pombe, is reversibly inhibited by the antimitotic agent Colcemid (N-deacetyl-N-methylcolchicine) in nutrient medium. Cell growth continues until all cells become nonseparating cell doublets in a V configuration. Mutants have been isolated capab...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1970-04, Vol.168 (3930), p.485-487
Hauptverfasser:	Lederberg, Seymour, Stetten, Gail
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells Cell culture techniques Cell division Cell Division - drug effects Cell growth Colchicine - pharmacology Cultured cells Drug Resistance, Microbial Haploidy Mutation Plant cells Protein synthesis Saccharomyces - drug effects Saccharomyces - growth & development Somatic cells Yeasts
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Beschreibung
Zusammenfassung:	Cell division of the fission yeast, Schizosaccharomyces pombe, is reversibly inhibited by the antimitotic agent Colcemid (N-deacetyl-N-methylcolchicine) in nutrient medium. Cell growth continues until all cells become nonseparating cell doublets in a V configuration. Mutants have been isolated capable of uninhibited growth in the presence of concentrations of Colcemid mycostatic for the parent strain.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.168.3930.485