An enzyme assay for norepinephrine in brain tissue

An enzyme assay for norepinephrine in brain tissue

An enzymatic assay for norepinephrine has been developed in which the methyl- 14C group of S-adenosylmethionine-methyl- 14C is transferred to the primary amine nitrogen of norepinephrine by the enzyme phenylethanolamme- N-methyltransferase. The rate of formation of the product, epinephrine- N-methyl...

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Veröffentlicht in:Biochemical pharmacology 1967-06, Vol.16 (6), p.1043-1049
Hauptverfasser: Saelens, J.K., Schoen, M.S., Kovacsics, G.B.
Format: Artikel
Sprache:eng
Schlagworte:
Alkylation
Animals
Brain - drug effects
Brain - metabolism
Carbon Isotopes
Methionine - metabolism
Mice
Norepinephrine - metabolism
Pargyline - pharmacology
Rabbits
Reserpine - pharmacology
Transferases - metabolism
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Zusammenfassung:An enzymatic assay for norepinephrine has been developed in which the methyl- 14C group of S-adenosylmethionine-methyl- 14C is transferred to the primary amine nitrogen of norepinephrine by the enzyme phenylethanolamme- N-methyltransferase. The rate of formation of the product, epinephrine- N-methyl- 14C, was linearly related to the concentration of substrate norepinephrine up to 5.0 μg/ml. The assay is specific for norepinephrine and can be used to measure this amine in as little as 10 mg of brain tissue.
ISSN:0006-2952
1873-2968
DOI:10.1016/0006-2952(67)90277-8