A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis

A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis

Protein zones may be stained by Coomassie blue without any destaining of the background and with a sensitivity at least as high as that of amido black. A vastly increased sensitivity may be achieved with Coomassie blue at the price of background destaining.

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Bibliographische Detailangaben
Veröffentlicht in:Analytical biochemistry 1967-07, Vol.20 (1), p.150-154
Hauptverfasser: Chrambach, A., Reisfeld, R.A., Wyckoff, M., Zaccari, J.
Format: Artikel
Sprache:eng
Schlagworte:
Acrylic Resins
Animals
Blood Proteins - analysis
Cattle
Electrophoresis
gamma-Globulins - analysis
Gels
Lectins - analysis
Parathyroid Hormone - analysis
Plant Proteins - analysis
Prolactin - analysis
Proteins - analysis
Rabbits
Staining and Labeling
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Beschreibung
Zusammenfassung:Protein zones may be stained by Coomassie blue without any destaining of the background and with a sensitivity at least as high as that of amido black. A vastly increased sensitivity may be achieved with Coomassie blue at the price of background destaining.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(67)90272-2