Fast Spectrophotometric Measurement of H + Changes in Chromatium Chromatophores activated by a Liquid Dye Laser

Fast Spectrophotometric Measurement of H + Changes in Chromatium Chromatophores activated by a Liquid Dye Laser

The fast changes in hydrogen ion concentration which follow photo-activation of the bacteriochlorophyll complex of Chromatium have been measured by observing absorbance changes of the p H indicator bromcresol purple using a ruby-pumped dye laser. This acts as an optical frequency converter with an o...

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Veröffentlicht in:Nature (London) 1970-02, Vol.225 (5234), p.705-708
Hauptverfasser: CHANCE, B, McCRAY, J. A, BUNKENBURG, J
Format: Artikel
Sprache:eng
Schlagworte:
Bacterial Chromatophores
Binding Sites
Chromatium
Coloring Agents
Humanities and Social Sciences
Hydrogen-Ion Concentration
Lasers
Light
Methods
multidisciplinary
Science
Science (multidisciplinary)
Spectrophotometry
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Beschreibung
Zusammenfassung:The fast changes in hydrogen ion concentration which follow photo-activation of the bacteriochlorophyll complex of Chromatium have been measured by observing absorbance changes of the p H indicator bromcresol purple using a ruby-pumped dye laser. This acts as an optical frequency converter with an output pulse corresponding to an absorption peak of the chromatophore. The changes in H + binding represent a sort of “membrane Bohr effect”.
ISSN:0028-0836
1476-4687
DOI:10.1038/225705a0