Optimal conditions for immunoelectrophoresis of some soluble bovine lens antigens

It was demonstrated that the conventional conditions for immunoelectrophoresis of lens antigens with respect to pH, temperature and specific conductance of buffer may not be adequate. The best results were obtained with a Tris-phosphate buffer with a specific conductance equivalent to that of 0·025 m KCl, at 4°C with a pH of 6·0–7·5. The optimum conditions, however, varied depending upon which lens antigen was being studied. Immunization of rabbits to bovine lens antigens was considerably more successful when relatively purified protein fractions were utilized rather than the total soluble lens protein fraction.