A new rapid electrophoretic method for determination of deoxyribonucleic acid base composition

A new rapid electrophoretic method for determination of deoxyribonucleic acid base composition

A method is presented for the rapid analysis of DNA base composition. After separation from RNA by alkaline hydrolysis a DNA preparation was hydrolyzed with 72% HClO 4 for 1 hr. After hydrolysis, HClO 4 was removed by evaporation under reduced pressure. Dry powder containing the bases was dissolved...

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Veröffentlicht in:Analytical biochemistry 1969, Vol.27 (1), p.58-64
Hauptverfasser: Borkowski, T., Wojcierowski, J., Kulesza, S.
Format: Artikel
Sprache:eng
Schlagworte:
Adenine
Agar
DNA - analysis
Electrophoresis
Guanine
Hydrogen-Ion Concentration
Methods
Nucleosides
Nucleotides - analysis
Perchlorates
Pressure
Spectrophotometry
Thymine
Time Factors
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Zusammenfassung:A method is presented for the rapid analysis of DNA base composition. After separation from RNA by alkaline hydrolysis a DNA preparation was hydrolyzed with 72% HClO 4 for 1 hr. After hydrolysis, HClO 4 was removed by evaporation under reduced pressure. Dry powder containing the bases was dissolved in water and separated by agar gel electrophoresis. The spots of each base were cut out with agar gel and after dissolution in warm water were determined spectrophotometrically. The method may be applied to determination of base composition in 50 μg of analyzed DNA.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(69)90218-8