Separation and Quantitative Determination of Adrenaline Using Thin-Layer Chromatography

The separation of adrenaline from noradrenaline and dopamine and its quantitative determination using thin-layer chromatography have been studied. A complete spot separation of the above amines can be achieved by the use of cellulose thin layers and a phenol-water solvent system. For the quantitative determination of the separated adrenaline two methods were used, namely (a) a method studying the weight/area relationship of the spots and (b) a method employing elution of the spots and measurement of the ultraviolet absorption of the eluents.