Use of phenazine methosulphate (PMS) in the histochemical localization of lactic acid dehydrogenase (LDH) in the retina

Lactic acid dehydrogenase activity has been investigated histochemically using tetrazolium staining. The importance of the inclusion of phenazine methosulphate is emphasized, and possible explanations for discrepancies in previous reports are suggested. In the presence of phenazine methosulphate, pr...

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Veröffentlicht in:	Experimental eye research 1969-10, Vol.8 (4), p.375,IN1,377-376,IN2,378
Hauptverfasser:	Graymore, Clive N., Kissun, Ralph D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Histocytochemistry Indicators and Reagents L-Lactate Dehydrogenase - analysis Phenazines Photoreceptor Cells - enzymology Rabbits Retina - enzymology Staining and Labeling Tetrazolium Salts
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Zusammenfassung:	Lactic acid dehydrogenase activity has been investigated histochemically using tetrazolium staining. The importance of the inclusion of phenazine methosulphate is emphasized, and possible explanations for discrepancies in previous reports are suggested. In the presence of phenazine methosulphate, precipitation induced by lactic acid dehydrogenase is most concentrated in the inner layers of the retina.
ISSN:	0014-4835
DOI:	10.1016/S0014-4835(69)80001-1