A rapid assay for cholesterol side-chain desmolase and the quantitative determination of desmolase activity in adrenals
Methods for reproducible extraction of soluble cholesterol side-chain desmolase from adrenal cortex and for rapid assay of this enzyme under optimal conditions are described. The desmolase activity of normal rat adrenal thus determined is 1.3±0.16 μmoles/hr/gm wet weight adrenal.
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| Veröffentlicht in: | Analytical biochemistry 1966-08, Vol.16 (2), p.355-358 |
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| container_end_page | 358 |
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| container_issue | 2 |
| container_start_page | 355 |
| container_title | Analytical biochemistry |
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| creator | Kimura, Tokuji Satoh, P.S. Tchen, T.T. |
| description | Methods for reproducible extraction of soluble cholesterol side-chain desmolase from adrenal cortex and for rapid assay of this enzyme under optimal conditions are described. The desmolase activity of normal rat adrenal thus determined is 1.3±0.16 μmoles/hr/gm wet weight adrenal. |
| doi_str_mv | 10.1016/0003-2697(66)90166-7 |
| format | Article |
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The desmolase activity of normal rat adrenal thus determined is 1.3±0.16 μmoles/hr/gm wet weight adrenal.</description><subject>Adrenal Glands - enzymology</subject><subject>Animals</subject><subject>Carbon Isotopes</subject><subject>Cholesterol</subject><subject>Lyases - analysis</subject><subject>Pregnenolone</subject><subject>Radiometry</subject><subject>Rats</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1966</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UE1rGzEQFaHBddL8gxZ0Ks1hU33sjlaXgAlJWzDkkp6FVprFKrsrR1on-N9Xrk3Iqadh5n0M7xHymbMbzjh8Z4zJSoBW3wCudblApc7IkjMNFZNMfyDLN8pHcpHzH8Y4rxtYkEWjASSIJXld0WS3wVObs93TPibqNnHAPGOKA83BY-U2NkzUYx7jYDNSO3k6b5A-7-w0h9nO4QULXBRjmMoWJxr793xXGGHe0-JifcLJDvkTOe_LwKvTvCS_H-6f7n5W68cfv-5W68oJJeZKC-awA96pXrWNtb2SvJUSmq4FJcCXrG1Xg_JCOG21UBJq1rG-0Z0HJ6W8JF-PvtsUn3cllhlDdjgMdsK4y6atJRdSs0Ksj0SXYs4Je7NNYbRpbzgzh77NoUxzKNMAmH99G1VkX07-u25E_yY6FVzw2yOOJeRLwGSyCzg59CGhm42P4f8P_gLLkJBb</recordid><startdate>196608</startdate><enddate>196608</enddate><creator>Kimura, Tokuji</creator><creator>Satoh, P.S.</creator><creator>Tchen, T.T.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196608</creationdate><title>A rapid assay for cholesterol side-chain desmolase and the quantitative determination of desmolase activity in adrenals</title><author>Kimura, Tokuji ; Satoh, P.S. ; Tchen, T.T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-920ceb61b7f785aaf73183365b86726d0968b467d22c9a9273640b0f59bd6c333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1966</creationdate><topic>Adrenal Glands - enzymology</topic><topic>Animals</topic><topic>Carbon Isotopes</topic><topic>Cholesterol</topic><topic>Lyases - analysis</topic><topic>Pregnenolone</topic><topic>Radiometry</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kimura, Tokuji</creatorcontrib><creatorcontrib>Satoh, P.S.</creatorcontrib><creatorcontrib>Tchen, T.T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kimura, Tokuji</au><au>Satoh, P.S.</au><au>Tchen, T.T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid assay for cholesterol side-chain desmolase and the quantitative determination of desmolase activity in adrenals</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1966-08</date><risdate>1966</risdate><volume>16</volume><issue>2</issue><spage>355</spage><epage>358</epage><pages>355-358</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Methods for reproducible extraction of soluble cholesterol side-chain desmolase from adrenal cortex and for rapid assay of this enzyme under optimal conditions are described. 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| fulltext | fulltext |
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| ispartof | Analytical biochemistry, 1966-08, Vol.16 (2), p.355-358 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84312390 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adrenal Glands - enzymology Animals Carbon Isotopes Cholesterol Lyases - analysis Pregnenolone Radiometry Rats |
| title | A rapid assay for cholesterol side-chain desmolase and the quantitative determination of desmolase activity in adrenals |
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