A rapid assay for cholesterol side-chain desmolase and the quantitative determination of desmolase activity in adrenals

A rapid assay for cholesterol side-chain desmolase and the quantitative determination of desmolase activity in adrenals

Methods for reproducible extraction of soluble cholesterol side-chain desmolase from adrenal cortex and for rapid assay of this enzyme under optimal conditions are described. The desmolase activity of normal rat adrenal thus determined is 1.3±0.16 μmoles/hr/gm wet weight adrenal.

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Veröffentlicht in:Analytical biochemistry 1966-08, Vol.16 (2), p.355-358
Hauptverfasser: Kimura, Tokuji, Satoh, P.S., Tchen, T.T.
Format: Artikel
Sprache:eng
Schlagworte:
Adrenal Glands - enzymology
Animals
Carbon Isotopes
Cholesterol
Lyases - analysis
Pregnenolone
Radiometry
Rats
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Zusammenfassung:Methods for reproducible extraction of soluble cholesterol side-chain desmolase from adrenal cortex and for rapid assay of this enzyme under optimal conditions are described. The desmolase activity of normal rat adrenal thus determined is 1.3±0.16 μmoles/hr/gm wet weight adrenal.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(66)90166-7