Enzyme resolution in starch gel electrophoresis

1. 1. Improved resolution of enzyme bands in starch gel electrophoresis obtained by assaying extracts of 1 mm slices is well worth the increased manipulative requirements, for research purposes. Resolution is also improved by limiting slab thickness to about 2 mm or less before cutting the slices. 2. 2. Starch gel electrophoresis of honey α-glucosidase preparations show up to ten distinct bands in a 30 mm zone of activity when processed in this manner; only one activity zone was found by direct staining of the gel by chromogenic substrate reagents.