A rapid radioassay technique for cellular suspensions
A simplified technique for studying in vivo the uptake of labeled precursors into proteins and nucleic acids of microorganisms is presented. Applications to studies in protozoan and bacterial systems are represented. The details of the technique are discussed along with drawbacks and advantages.
Gespeichert in:
Veröffentlicht in: | Analytical biochemistry 1966-12, Vol.17 (3), p.434-443 |
---|---|
Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 443 |
---|---|
container_issue | 3 |
container_start_page | 434 |
container_title | Analytical biochemistry |
container_volume | 17 |
creator | Byfield, John E. Scherbaum, Otto H. |
description | A simplified technique for studying
in vivo the uptake of labeled precursors into proteins and nucleic acids of microorganisms is presented. Applications to studies in protozoan and bacterial systems are represented. The details of the technique are discussed along with drawbacks and advantages. |
doi_str_mv | 10.1016/0003-2697(66)90179-5 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_84299309</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0003269766901795</els_id><sourcerecordid>84299309</sourcerecordid><originalsourceid>FETCH-LOGICAL-c423t-96417a67d534adde628b4c8039acf49d51382a076af6c1e66d7ddb7f346f018d3</originalsourceid><addsrcrecordid>eNp9kE9LxDAQxYMo67r6DRR6Ej1UJ00ybS7CsvgPFrzoOaRJipFuW5NW2G9v6y579DJzmDdv3vwIuaRwR4HiPQCwNEOZ3yDeSqC5TMURmVOQmAIDeUzmB8kpOYvxC4BSLnBGZoKxAgSbE7FMgu68Hav1rY5Rb5Pemc_Gfw8uqdqQGFfXQ61DEofYuSb6tonn5KTSdXQX-74gH0-P76uXdP32_LparlPDM9anEjnNNeZWMK6tdZgVJTcFMKlNxaUVlBWZhhx1hYY6RJtbW-YV41gBLSxbkOudbxfaMU_s1cbHKZBuXDtEVfBMyvHVUch3QhPaGIOrVBf8RoetoqAmWmpCoSYUClH90VJiXLva-w_lxtnD0h7POH_Yzd345I93QUXjXWOc9cGZXtnW_3_gF8hveHs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>84299309</pqid></control><display><type>article</type><title>A rapid radioassay technique for cellular suspensions</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Byfield, John E. ; Scherbaum, Otto H.</creator><creatorcontrib>Byfield, John E. ; Scherbaum, Otto H.</creatorcontrib><description>A simplified technique for studying
in vivo the uptake of labeled precursors into proteins and nucleic acids of microorganisms is presented. Applications to studies in protozoan and bacterial systems are represented. The details of the technique are discussed along with drawbacks and advantages.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(66)90179-5</identifier><identifier>PMID: 5338053</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acids - metabolism ; Buffers ; Carbon Isotopes ; Culture Media ; Escherichia coli - metabolism ; Indicators and Reagents ; Protein Biosynthesis ; Radiometry ; RNA - biosynthesis ; RNA, Bacterial - biosynthesis ; Suspensions ; Tetrahymena - metabolism ; Uracil - metabolism</subject><ispartof>Analytical biochemistry, 1966-12, Vol.17 (3), p.434-443</ispartof><rights>1966</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-96417a67d534adde628b4c8039acf49d51382a076af6c1e66d7ddb7f346f018d3</citedby><cites>FETCH-LOGICAL-c423t-96417a67d534adde628b4c8039acf49d51382a076af6c1e66d7ddb7f346f018d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(66)90179-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5338053$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Byfield, John E.</creatorcontrib><creatorcontrib>Scherbaum, Otto H.</creatorcontrib><title>A rapid radioassay technique for cellular suspensions</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A simplified technique for studying
in vivo the uptake of labeled precursors into proteins and nucleic acids of microorganisms is presented. Applications to studies in protozoan and bacterial systems are represented. The details of the technique are discussed along with drawbacks and advantages.</description><subject>Amino Acids - metabolism</subject><subject>Buffers</subject><subject>Carbon Isotopes</subject><subject>Culture Media</subject><subject>Escherichia coli - metabolism</subject><subject>Indicators and Reagents</subject><subject>Protein Biosynthesis</subject><subject>Radiometry</subject><subject>RNA - biosynthesis</subject><subject>RNA, Bacterial - biosynthesis</subject><subject>Suspensions</subject><subject>Tetrahymena - metabolism</subject><subject>Uracil - metabolism</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1966</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LxDAQxYMo67r6DRR6Ej1UJ00ybS7CsvgPFrzoOaRJipFuW5NW2G9v6y579DJzmDdv3vwIuaRwR4HiPQCwNEOZ3yDeSqC5TMURmVOQmAIDeUzmB8kpOYvxC4BSLnBGZoKxAgSbE7FMgu68Hav1rY5Rb5Pemc_Gfw8uqdqQGFfXQ61DEofYuSb6tonn5KTSdXQX-74gH0-P76uXdP32_LparlPDM9anEjnNNeZWMK6tdZgVJTcFMKlNxaUVlBWZhhx1hYY6RJtbW-YV41gBLSxbkOudbxfaMU_s1cbHKZBuXDtEVfBMyvHVUch3QhPaGIOrVBf8RoetoqAmWmpCoSYUClH90VJiXLva-w_lxtnD0h7POH_Yzd345I93QUXjXWOc9cGZXtnW_3_gF8hveHs</recordid><startdate>196612</startdate><enddate>196612</enddate><creator>Byfield, John E.</creator><creator>Scherbaum, Otto H.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196612</creationdate><title>A rapid radioassay technique for cellular suspensions</title><author>Byfield, John E. ; Scherbaum, Otto H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-96417a67d534adde628b4c8039acf49d51382a076af6c1e66d7ddb7f346f018d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1966</creationdate><topic>Amino Acids - metabolism</topic><topic>Buffers</topic><topic>Carbon Isotopes</topic><topic>Culture Media</topic><topic>Escherichia coli - metabolism</topic><topic>Indicators and Reagents</topic><topic>Protein Biosynthesis</topic><topic>Radiometry</topic><topic>RNA - biosynthesis</topic><topic>RNA, Bacterial - biosynthesis</topic><topic>Suspensions</topic><topic>Tetrahymena - metabolism</topic><topic>Uracil - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Byfield, John E.</creatorcontrib><creatorcontrib>Scherbaum, Otto H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Byfield, John E.</au><au>Scherbaum, Otto H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid radioassay technique for cellular suspensions</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1966-12</date><risdate>1966</risdate><volume>17</volume><issue>3</issue><spage>434</spage><epage>443</epage><pages>434-443</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A simplified technique for studying
in vivo the uptake of labeled precursors into proteins and nucleic acids of microorganisms is presented. Applications to studies in protozoan and bacterial systems are represented. The details of the technique are discussed along with drawbacks and advantages.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>5338053</pmid><doi>10.1016/0003-2697(66)90179-5</doi><tpages>10</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0003-2697 |
ispartof | Analytical biochemistry, 1966-12, Vol.17 (3), p.434-443 |
issn | 0003-2697 1096-0309 |
language | eng |
recordid | cdi_proquest_miscellaneous_84299309 |
source | MEDLINE; Elsevier ScienceDirect Journals |
subjects | Amino Acids - metabolism Buffers Carbon Isotopes Culture Media Escherichia coli - metabolism Indicators and Reagents Protein Biosynthesis Radiometry RNA - biosynthesis RNA, Bacterial - biosynthesis Suspensions Tetrahymena - metabolism Uracil - metabolism |
title | A rapid radioassay technique for cellular suspensions |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T15%3A55%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20rapid%20radioassay%20technique%20for%20cellular%20suspensions&rft.jtitle=Analytical%20biochemistry&rft.au=Byfield,%20John%20E.&rft.date=1966-12&rft.volume=17&rft.issue=3&rft.spage=434&rft.epage=443&rft.pages=434-443&rft.issn=0003-2697&rft.eissn=1096-0309&rft_id=info:doi/10.1016/0003-2697(66)90179-5&rft_dat=%3Cproquest_cross%3E84299309%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=84299309&rft_id=info:pmid/5338053&rft_els_id=0003269766901795&rfr_iscdi=true |