A rapid radioassay technique for cellular suspensions

A simplified technique for studying in vivo the uptake of labeled precursors into proteins and nucleic acids of microorganisms is presented. Applications to studies in protozoan and bacterial systems are represented. The details of the technique are discussed along with drawbacks and advantages.

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Veröffentlicht in:Analytical biochemistry 1966-12, Vol.17 (3), p.434-443
Hauptverfasser: Byfield, John E., Scherbaum, Otto H.
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container_title Analytical biochemistry
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creator Byfield, John E.
Scherbaum, Otto H.
description A simplified technique for studying in vivo the uptake of labeled precursors into proteins and nucleic acids of microorganisms is presented. Applications to studies in protozoan and bacterial systems are represented. The details of the technique are discussed along with drawbacks and advantages.
doi_str_mv 10.1016/0003-2697(66)90179-5
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source MEDLINE; Elsevier ScienceDirect Journals
subjects Amino Acids - metabolism
Buffers
Carbon Isotopes
Culture Media
Escherichia coli - metabolism
Indicators and Reagents
Protein Biosynthesis
Radiometry
RNA - biosynthesis
RNA, Bacterial - biosynthesis
Suspensions
Tetrahymena - metabolism
Uracil - metabolism
title A rapid radioassay technique for cellular suspensions
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