A rapid radioassay technique for cellular suspensions

A rapid radioassay technique for cellular suspensions

A simplified technique for studying in vivo the uptake of labeled precursors into proteins and nucleic acids of microorganisms is presented. Applications to studies in protozoan and bacterial systems are represented. The details of the technique are discussed along with drawbacks and advantages.

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Veröffentlicht in:Analytical biochemistry 1966-12, Vol.17 (3), p.434-443
Hauptverfasser: Byfield, John E., Scherbaum, Otto H.
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acids - metabolism
Buffers
Carbon Isotopes
Culture Media
Escherichia coli - metabolism
Indicators and Reagents
Protein Biosynthesis
Radiometry
RNA - biosynthesis
RNA, Bacterial - biosynthesis
Suspensions
Tetrahymena - metabolism
Uracil - metabolism
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Zusammenfassung:A simplified technique for studying in vivo the uptake of labeled precursors into proteins and nucleic acids of microorganisms is presented. Applications to studies in protozoan and bacterial systems are represented. The details of the technique are discussed along with drawbacks and advantages.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(66)90179-5