Effect of Dilution During Fractionation and Extraction of Serum and Lymph Insulin.

Summary An increase in ILA has been shown after gel nitration of rat serum and lymph and after acid-alcohol extraction of guinea pig serum. This effect was reproduced simply by a dilution-reconcentration step, common to both procedures. It is conceivable that a similar step is responsible for the increment of ILA observed by other investigators using many different techniques. The mechanism by which the dilution-reconcentration procedure enhances ILA is not clear; its physiological role, if any, is dubious, and theories on the state of insulin should be formulated with caution.