The Complete Amino Acid Sequence of Two Types of Subtilisin, BPN' and Carlsberg

The complete amino acid sequences of subtilisins BPN' (Nagarse) and Carlsberg have been determined. Subtilisin BPN' consists of a single peptide chain of 275 amino acid residues, whereas the Carlsberg enzyme contains 274 residues and differs in a total of 83 residues. The enzymes are distinct from "serine proteinases" of animal origin. Amino acid replacements permit preliminary identification of areas critical for structure-function relationships and some assessment of possible genetic mechanisms which have led to differences in sequence.