An enzyme assay for acetylcholine

An enzyme assay for acetylcholine

A specific and sensitive enzymatic assay for acetylcholine has been developed. The acetylcholine was isolated from tissue extracts by electrophoresis, hydrolyzed to choline, and reacetylated by the transfer of the acetyl- 14C group of acetyl- 14C-coenzyme A to the choline molecule by the enzyme, cho...

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Veröffentlicht in:Biochemical pharmacology 1969-06, Vol.18 (6), p.1479,in5,1481-1480,in6,1486
Hauptverfasser: Feigenson, M.E., Saelens, J.K.
Format: Artikel
Sprache:eng
Schlagworte:
Acetates - metabolism
Acetylcholine - analysis
Acyltransferases
Animals
Brain Chemistry
Carbon Isotopes
Choline
Coenzyme A
Electrophoresis
Kinetics
Male
Methods
Mice
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Beschreibung
Zusammenfassung:A specific and sensitive enzymatic assay for acetylcholine has been developed. The acetylcholine was isolated from tissue extracts by electrophoresis, hydrolyzed to choline, and reacetylated by the transfer of the acetyl- 14C group of acetyl- 14C-coenzyme A to the choline molecule by the enzyme, choline acetyltransferase. The rate of formation of the product, acetyl- 14C-cholinc, was linearly related to the concentration of substrate choline up to 30.0 μg per ml. The assay can be used to measure acetylcholine in as little as 10 mg brain tissue (0.01 μg acetylcholine).
ISSN:0006-2952
1873-2968
DOI:10.1016/0006-2952(69)90262-7