Estimation of adenine nucleotides in brain by enzymic and ion-exchange chromatographic methods

Estimation of adenine nucleotides in brain by enzymic and ion-exchange chromatographic methods

A spectrophotometric assay for the adenine nucleotides using AMP deaminase, myokinase (MK) and apyrase has been examined and found to give erroneously high estimates of AMP, ADP and ATP in rat brain extract. The AMP deaminase preparation used is liable to contain creatine phosphokinase (CPK) and lac...

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Veröffentlicht in:Biochemical pharmacology 1969-06, Vol.18 (6), p.1297-1306
Hauptverfasser: Wilson, W.S., Thomson, R.Y.
Format: Artikel
Sprache:eng
Schlagworte:
Adenine Nucleotides - analysis
Adenosine Triphosphatases
Aminohydrolases
Animals
Brain Chemistry
Chromatography, Ion Exchange
Creatine Kinase
Freeze Drying
Freezing
L-Lactate Dehydrogenase
Male
Methods
Muscles
Phosphotransferases
Rats
Solvents
Spectrophotometry
Water
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Zusammenfassung:A spectrophotometric assay for the adenine nucleotides using AMP deaminase, myokinase (MK) and apyrase has been examined and found to give erroneously high estimates of AMP, ADP and ATP in rat brain extract. The AMP deaminase preparation used is liable to contain creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) activities. However, it is suggested that the discrepancy may be due to the presence of yet another enzymic contaminant. Some aspects of nucleotide extraction from brain tissue are investigated.
ISSN:0006-2952
1873-2968
DOI:10.1016/0006-2952(69)90242-1