Sucrose interference in the assay of enzymes and protein
Sucrose has been found to interfere with the estimation of protein and with the assay of a variety of soluble and particulate enzymes. No interference was detected in the estimation of inorganic phosphate by the Lowry and Lopez procedure. The usual pattern of interference was a reversible reduction...
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| Veröffentlicht in: | Analytical biochemistry 1969-05, Vol.29 (2), p.248-256 |
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| container_end_page | 256 |
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| container_issue | 2 |
| container_start_page | 248 |
| container_title | Analytical biochemistry |
| container_volume | 29 |
| creator | Hinton, R.H. Burge, M.L.E. Hartman, G.C. |
| description | Sucrose has been found to interfere with the estimation of protein and with the assay of a variety of soluble and particulate enzymes. No interference was detected in the estimation of inorganic phosphate by the Lowry and Lopez procedure. The usual pattern of interference was a reversible reduction of activity, linearly related to sucrose concentration. |
| doi_str_mv | 10.1016/0003-2697(69)90308-X |
| format | Article |
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No interference was detected in the estimation of inorganic phosphate by the Lowry and Lopez procedure. 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No interference was detected in the estimation of inorganic phosphate by the Lowry and Lopez procedure. The usual pattern of interference was a reversible reduction of activity, linearly related to sucrose concentration.</description><subject>Alcohol Oxidoreductases - analysis</subject><subject>Centrifugation, Density Gradient</subject><subject>Centrifugation, Zonal</subject><subject>Liver</subject><subject>Phosphates - analysis</subject><subject>Phosphoric Monoester Hydrolases - analysis</subject><subject>Ribonucleases - analysis</subject><subject>Serum Albumin, Bovine - analysis</subject><subject>Succinate Dehydrogenase - analysis</subject><subject>Sucrose</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1969</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1Lw0AQxRdRaq3-Bwo5iR6is9nNflyEUvyCggcVelu2mwmuNEndTYT615vY0qOn4THvzfB-hJxTuKFAxS0AsDQTWl4Jfa2BgUoXB2RMQYu0V_qQjPeWY3IS4ycApTwXIzLiDCQDOibqtXOhiZj4usVQYsDaDSJpPzCxMdpN0pQJ1j-bCmNi6yJZh6ZFX5-So9KuIp7t5oS8P9y_zZ7S-cvj82w6Tx3LZZvqTHAllCwxVzJ3UoIQDmgml8JhQVmmUTpNM5VDkZUWcxSccYu6cBJAUTYhl9u7_d-vDmNrKh8drla2xqaLRnGqBSjojXxrHPrEgKVZB1_ZsDEUzADMDDTMQMMIbf6AmUUfu9jd75YVFvvQjlC_v9vusS_57TGY6PwAqfABXWuKxv__4Bd9P3ij</recordid><startdate>196905</startdate><enddate>196905</enddate><creator>Hinton, R.H.</creator><creator>Burge, M.L.E.</creator><creator>Hartman, G.C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196905</creationdate><title>Sucrose interference in the assay of enzymes and protein</title><author>Hinton, R.H. ; Burge, M.L.E. ; Hartman, G.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-92648687fe5875c77066c0127b6ced1329e7c912850d2fae5e6434ae9dc700813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1969</creationdate><topic>Alcohol Oxidoreductases - analysis</topic><topic>Centrifugation, Density Gradient</topic><topic>Centrifugation, Zonal</topic><topic>Liver</topic><topic>Phosphates - analysis</topic><topic>Phosphoric Monoester Hydrolases - analysis</topic><topic>Ribonucleases - analysis</topic><topic>Serum Albumin, Bovine - analysis</topic><topic>Succinate Dehydrogenase - analysis</topic><topic>Sucrose</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hinton, R.H.</creatorcontrib><creatorcontrib>Burge, M.L.E.</creatorcontrib><creatorcontrib>Hartman, G.C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hinton, R.H.</au><au>Burge, M.L.E.</au><au>Hartman, G.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sucrose interference in the assay of enzymes and protein</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1969-05</date><risdate>1969</risdate><volume>29</volume><issue>2</issue><spage>248</spage><epage>256</epage><pages>248-256</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Sucrose has been found to interfere with the estimation of protein and with the assay of a variety of soluble and particulate enzymes. No interference was detected in the estimation of inorganic phosphate by the Lowry and Lopez procedure. The usual pattern of interference was a reversible reduction of activity, linearly related to sucrose concentration.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4307301</pmid><doi>10.1016/0003-2697(69)90308-X</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1969-05, Vol.29 (2), p.248-256 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84196080 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Alcohol Oxidoreductases - analysis Centrifugation, Density Gradient Centrifugation, Zonal Liver Phosphates - analysis Phosphoric Monoester Hydrolases - analysis Ribonucleases - analysis Serum Albumin, Bovine - analysis Succinate Dehydrogenase - analysis Sucrose |
| title | Sucrose interference in the assay of enzymes and protein |
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