Sucrose interference in the assay of enzymes and protein

Sucrose has been found to interfere with the estimation of protein and with the assay of a variety of soluble and particulate enzymes. No interference was detected in the estimation of inorganic phosphate by the Lowry and Lopez procedure. The usual pattern of interference was a reversible reduction...

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Veröffentlicht in:	Analytical biochemistry 1969-05, Vol.29 (2), p.248-256
Hauptverfasser:	Hinton, R.H., Burge, M.L.E., Hartman, G.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcohol Oxidoreductases - analysis Centrifugation, Density Gradient Centrifugation, Zonal Liver Phosphates - analysis Phosphoric Monoester Hydrolases - analysis Ribonucleases - analysis Serum Albumin, Bovine - analysis Succinate Dehydrogenase - analysis Sucrose
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Beschreibung
Zusammenfassung:	Sucrose has been found to interfere with the estimation of protein and with the assay of a variety of soluble and particulate enzymes. No interference was detected in the estimation of inorganic phosphate by the Lowry and Lopez procedure. The usual pattern of interference was a reversible reduction of activity, linearly related to sucrose concentration.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(69)90308-X