Enhancement of Lysozyme Activity by Anodal Tear Protein

Summary Anodal tear protein is a constituent of human and rabbit conjunctival fluid. This protein has not been identified in other body fluids. Rabbit anodal tear protein enhances the lysis of M. lysodeikticus by human and egg white lysozyme. Neither human serum albumin nor an acidic derivative of serum albumin enhanced the activity of lysozyme. The enhancing action of the acidic tear protein was noted when the assay was performed in 0.01 M phosphate buffer, pH 7.0, but was not noted when 0.1 M phosphate buffer was used.