Synthesis of estrogen glutathione and cysteine derivatives

The compound S-(2,3,17β-trihydroxy-1,3,5(10)-estratrien-4-(or 1)-yl)-glutathione has been synthesized from 2-hydroxyestradiol and glutathione and its structure established by nmr and ultraviolet spectra as well as from its chemical properties. The synthetic compound had properties identical to those reported by other workers for biosynthetic material derived from liver incubations. The corresponding S-(2-hydroxyestradiol)-L-cysteine and N-acetyl-S-(2-hydroxyestradiol)-L-cysteine compounds have also been synthesized.