Detection of Soluble Antigen of Epstein-Barr Virus by the Enzyme-Linked Immunosorbent Assay

The enzyme-linked immunosorbent assay (ELISA) was compared with the micro-complement-fixation test for sensitivity and reliability in detection of antibody to soluble antigen of Epstein-Barr virus. ELISA was found to be more sensitive (up to 160 times more antibody) in detecting levels of antibody t...

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Veröffentlicht in:	The Journal of infectious diseases 1977-10, Vol.136 (Supplement-2), p.S324-S328
Hauptverfasser:	Wallen, William C., Mattson, Janet M., Levine, Paul H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Antibodies, Viral Antigens Antigens, Viral - isolation & purification Burkitt lymphoma Capsid - immunology Carcinoma Cell lines Complement Fixation Tests Enzyme linked immunosorbent assay Epstein Barr virus infections Herpesvirus 4, Human - immunology Human herpesvirus 4 Humans Infectious mononucleosis Scientific Articles Viruses
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Beschreibung
Zusammenfassung:	The enzyme-linked immunosorbent assay (ELISA) was compared with the micro-complement-fixation test for sensitivity and reliability in detection of antibody to soluble antigen of Epstein-Barr virus. ELISA was found to be more sensitive (up to 160 times more antibody) in detecting levels of antibody than the complement- fixation assay. The ELISA test was shown also to be comparably reliable to the complement-fixation assay in detecting immunity to this antigen; only one false-positive response was found in tests of 21 sera. Because this assay is completely quantifiable, it provides an easy, rapid, and highly sensitive technique for the detection of antibody to antigen of Epstein-Barr virus.
ISSN:	0022-1899 1537-6613
DOI:	10.1093/infdis/136.Supplement_2.S324