Thermal stability of T2 DNA in situ

Thermal stability of T2 DNA in situ

Thermal denaturation experiments were carried out on solutions of formaldehydefixed T2 bacteriophage and purified T2 DNA at low salt concentrations. Measurements of the flow birefringence, ultraviolet absorbance, and sedimentation velocity indicated that the tertiary structure of the internal DNA re...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 1969-06, Vol.38 (2), p.269-277
Hauptverfasser: Inners, Daniel, Bendet, Irwin J.
Format: Artikel
Sprache:eng
Schlagworte:
Coliphages
DNA, Viral
Formaldehyde
Hot Temperature
Nucleic Acid Denaturation
Optics and Photonics
Ultracentrifugation
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Zusammenfassung:Thermal denaturation experiments were carried out on solutions of formaldehydefixed T2 bacteriophage and purified T2 DNA at low salt concentrations. Measurements of the flow birefringence, ultraviolet absorbance, and sedimentation velocity indicated that the tertiary structure of the internal DNA remains intact even at temperatures up to 20 ° higher than the melting temperature of free DNA under comparable conditions.
ISSN:0042-6822
1096-0341
DOI:10.1016/0042-6822(69)90368-7