Spray technique for applying stripmix (cellulose acetate) to tlc plates for tritiated organic compounds

A modification of the recent rolling technique of Redgwell, R. J., Turner, N A., and Bieleski, R. L. [(1974) J. Chromatogr. 88, 25–31] for stripping thin layers from chromatographic plates for radioactive measurements has been developed. Use of this rolling technique in the case of tritiated benzo[α]pyrene and metabolites yields erratic results. By the simple expedient of spraying (instead of rolling) of the Stripmix, highly reproducible data were obtained.