ISOLATION AND CHARACTERIZATION OF CARCINOSTATIC LIVER FACTORS ACTIVE IN VITRO

Bovine liver was fractionated for the carcinostatic factor active in vitro, using the original assay method of Nakahara and Fukuoka. Starting with 75% ethanol extract, from which basic components were removed by acidic resin column chromatography, further fractionations by means of acetone extraction and DEAE-cellulose chromatography showed that the activity may be due not to a single substance but to any one of several common constituents of normal liver, and the results of thin-layer chromatography with active fractions strongly indicated that they consist of simple sugars. Several hexoses and other sugars were then tested and many were found to show the expected activity.