Staining of vaccinia antigen by immunouranium technique

In a preliminary study on the localization of vaccinia antigen by immunouranium technique it appeared that most antigenic reactivity was destroyed during fixation and embedding. Nevertheless, viral particles were specifically localized 14 and 20 hours after infection of HeLa cells. They appeared in...

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Veröffentlicht in:	Experimental and molecular pathology 1965-02, Vol.4 (1), p.126-129
Hauptverfasser:	Donati, Edward J., Figge, Frank H.J., Sternberger, Ludwig A.
Format:	Artikel
Sprache:	eng
Schlagworte:	ANIMAL CELLS ANTIGENS BIOLOGY AND MEDICINE CHEMICAL REACTIONS Coloring Agents DYES ELECTRON MICROSCOPY Electrons ENVIRONMENT EXPANSION HELA CELLS Humans IMMUNITY Inclusion Bodies Microscopy Microscopy, Electron Old Medline PARTICLES PREPARATION Staining and Labeling Tissue Culture Techniques TISSUE CULTURES TISSUES URANIUM Vaccinia Vaccinia virus VACCINIA VIRUSES VIRUSES
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Zusammenfassung:	In a preliminary study on the localization of vaccinia antigen by immunouranium technique it appeared that most antigenic reactivity was destroyed during fixation and embedding. Nevertheless, viral particles were specifically localized 14 and 20 hours after infection of HeLa cells. They appeared in the cytoplasm either as scattered clusters or as discrete, dense inclusion bodies.
ISSN:	0014-4800 1096-0945
DOI:	10.1016/0014-4800(65)90028-6