A new method for qualitative and quantitative determination of di- and polyamines in animal tissues by gas-liquid chromatography

A new method for qualitative and quantitative determination of di- and polyamines in animal tissues by gas-liquid chromatography

A gas chromatography method for qualitative and quantitative determination of di- and polyamines from animal tissues was developed. The polyamines were extracted either from tissue homogenates or from standard solutions with the butanol method, opportunely modified in order to improve both the recov...

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Veröffentlicht in:Analytical biochemistry 1977-05, Vol.80 (1), p.101-107
Hauptverfasser: Beninati, Simone, Sartori, Claudia, Argento-Ceru, Maria Paola
Format: Artikel
Sprache:eng
Schlagworte:
Amines - analysis
Animals
Brain Chemistry
Cadaverine - analysis
Chromatography, Gas - methods
Liver - analysis
Male
Organ Specificity
Pancreas - analysis
Polyamines - analysis
Prostate - analysis
Putrescine - analysis
Rats
Spermidine - analysis
Spermine - analysis
Testis - analysis
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Zusammenfassung:A gas chromatography method for qualitative and quantitative determination of di- and polyamines from animal tissues was developed. The polyamines were extracted either from tissue homogenates or from standard solutions with the butanol method, opportunely modified in order to improve both the recoveries and the reproducibility of the results. Chromatography was performed on a GLC column prepared with Corning glass beads coated with 1% KOH and 4‰ Carbowax 20 M, with d-amphetamine and benzoamphetamine as internal standards. This column permits a good separation and a nearly complete recovery of di- and polyamines.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(77)90629-7