The effects of different methods of washing, drying and sterilizing glass surfaces on cell attachment and growth behavior

1. 1. The growth behaviour of HeLa cells in different human sera is greatly influenced by the treatment of the glass surface on which the cells grow. 2. 2. The attachment rate of HeLa cells to a glass surface is altered by the way in which the surface has been dried and sterilized. 3. 3. The possible physical explanation is briefly discussed. The differences reported may be ascribable to variations in the electrical charge on the differently treated glass surfaces. The difference in attachment may also be due to the possibility that different amount of serum proteins or attachment inhibiting principle in serum is adsorbed onto glass surfaces differently treated. 4. 4. Great care must be taken in the evaluation of cell attachment experiments, as many known and unknown factors can affect the results.