The location of the polio genome protein in viral RNAs and its implication for RNA synthesis

Evidence is presented that a small protein (VPg) is covalently attached to the 5′-terminal oligonucleotide VPg–pU-U–A–A–A–A–C–A–Gp of the polio genome, to nascent strands of the polio replicative intermediate and to poly(U) of minus strands. A model of polio RNA replication is proposed implicating V...

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Veröffentlicht in:	Nature (London) 1977-07, Vol.268 (5617), p.208-213
Hauptverfasser:	Nomoto, Akio, Detjen, Barbara, Pozzatti, Rudy, Wimmer, Eckard
Format:	Artikel
Sprache:	eng
Schlagworte:	Humanities and Social Sciences multidisciplinary Oligonucleotides - isolation & purification Poliovirus - metabolism Protein Binding RNA, Messenger - metabolism RNA, Viral - biosynthesis RNA, Viral - metabolism Science Science (multidisciplinary) Viral Proteins - metabolism Virus Replication
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Beschreibung
Zusammenfassung:	Evidence is presented that a small protein (VPg) is covalently attached to the 5′-terminal oligonucleotide VPg–pU-U–A–A–A–A–C–A–Gp of the polio genome, to nascent strands of the polio replicative intermediate and to poly(U) of minus strands. A model of polio RNA replication is proposed implicating VPg in initiation of RNA synthesis, possibly as primer.
ISSN:	0028-0836 1476-4687
DOI:	10.1038/268208a0