Mechanism of Carbon Isotope Fractionation Associated with Lipid Synthesis

The low carbon-13/carbon-12 ratio of lipids is shown to result from isotopic fractionation during the oxidation of pyruvate to acetyl coenzyme A. In vitro analysis of the kinetic isotope effects of this reaction indicates that there will be a large, temperature-dependent difference in the carbon-13/...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1977-07, Vol.197 (4300), p.261-263
Hauptverfasser:	DeNiro, Michael J., Epstein, Samuel
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetates Acetates - metabolism Atoms Carbon dioxide Carbon Isotopes - metabolism Carboxy-Lyases - metabolism Chemical composition Escherichia coli - metabolism Fractionation Glucose - metabolism Isotope effects Kinetic isotope effect Kinetics Lipid metabolism Lipids Lipids - biosynthesis Pyruvate Decarboxylase - metabolism Pyruvates - metabolism Saccharomyces cerevisiae - enzymology Sodium
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Beschreibung
Zusammenfassung:	The low carbon-13/carbon-12 ratio of lipids is shown to result from isotopic fractionation during the oxidation of pyruvate to acetyl coenzyme A. In vitro analysis of the kinetic isotope effects of this reaction indicates that there will be a large, temperature-dependent difference in the carbon-13/carbon-12 ratio between the methyl and carbonyl carbon atoms of acetyl coenzyme A and between those carbon atoms of lipid components which derive from them.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.327543