Resolution of components in sedimentation equilibrium concentration distributions

A procedure is discussed whereby the concentration distribution at sedimentation equilibrium may be resolved into the contributing redistributed components in the system. The procedure is shown to deal best with heterogeneous systems, but can also lend itself to the analysis of a system where the molecular weights of interacting proteins are quite similar by making composition constraints. In those cases where interaction occurs between components of the system, a calculation of the equilibrium constant which described the association can be made. Moreover, the fitting error is related to the fitting parameters in such a manner as to yield the estimated error of the equilibrium constant.