Electrophoretic study of the proteins of the hemolymph, intestine, excrement (and ova) of active and dispausal Dermacentor marginatus Sulz (Ixodidae) females

The polyacrylamide-gel electrophoresis was used to separate the negative charged proteins from adult females of the tick Dermacentor marginatus Sulz. There were separated up to 25 protein bands from haemolymph and up to 19 bands from midgut extracts of the engorged females. The number of protein bands in excreta and eggs was 15 and 18 respectively. Haemoproteins from haemolymph, midgut and eggs were different in their electrophoretic mobility. Active and diapausing ticks showed essential differences in protein spectra and content of haemolymph (the enrichment of them was characteristic of diapausing ticks). The presence or absence of reproductive diapause affects the digestive functions and has no influence on the excretory functions of the midgut.