A Defined Maintenance Medium for Supporting Chick Fibroblast Monolayers and for Plaque Formation by Venezuelan and Eastern Equine Encephalitis Viruses

A Defined Maintenance Medium for Supporting Chick Fibroblast Monolayers and for Plaque Formation by Venezuelan and Eastern Equine Encephalitis Viruses

A defined maintenance medium that supports chick fibroblast (CF) cell monolayers in a viable state for at least 10 days and permits the formation of plaques by Venezuelan (VEE) and Eastern (EEE) equine encephalitis viruses is described. The medium consists of a balanced salts solution, glucose, 2 am...

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Veröffentlicht in:The Journal of infectious diseases 1965-02, Vol.115 (1), p.77-82
1. Verfasser: Zebovitz, Eugene
Format: Artikel
Sprache:eng
Schlagworte:
Agar
Amino Acids
Animals
Bicarbonates
Cell growth
Chick Embryo
Chlorides
Culture Media
Cystine
Embryonic cells
Encephalitis Virus, Eastern Equine
Encephalitis Viruses
Encephalomyelitis, Eastern Equine
Fibroblasts
Glucose
Histidine
Kidney cells
Old Medline
Pharmacology
Sodium
Tissue Culture Techniques
Venezuelan equine encephalomyelitis
Viability
Viruses
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Zusammenfassung:A defined maintenance medium that supports chick fibroblast (CF) cell monolayers in a viable state for at least 10 days and permits the formation of plaques by Venezuelan (VEE) and Eastern (EEE) equine encephalitis viruses is described. The medium consists of a balanced salts solution, glucose, 2 amino acids (L-cystine and L-histidine . HCI), sodium bicarbonate, and agar. Plaque counts by VEE and EEE viruses on CF monolayers overlaid with this medium compared favorably with those obtained on a complex overlay medium.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/115.1.77