Distribution of secreted aspartyl proteinases using a polymerase chain reaction assay withSAP specific primers inCandida albicans isolates

Secreted aspartyl proteinase (Sap) distribution among differentC. albicans isolates was determined usingSAP-specific primers in polymerase chain reaction (PCR) assay.SAP1, SAP2, andSAP3 were detected in 13 of 40 (32.5 %),SAP4 in 38/40 (95 %),SAP5 were detected in 30/40 (75 %),SAP6 in 23/40 (57.5 %) ofC. albicans strains isolated from blood cultures.SAP1-SAP3 were detected in 37 of 40 (92.5 %),SAP4 were detected in 3/40 (7.5 %),SAP5 in 3/40 (7.5 %),SAP6 in 5/40 (12.5 %) ofC. albicans strains isolated from vaginal swab cultures. Sap1, Sap2 and Sap3 isoenzymes were found to be related to the vaginopathic potential ofC. albicans, Sap4, Sap5 and Sap6 isoenzymes were found to be correlated with systemic infections.