A reliable method to concentrate circulating DNA

Concentration of circulating DNA probes is required to increase the amount of DNA involved in subsequent study (by polymerase chain reaction, sequencing, and microarray). This work was dedicated to the comparison of five different methods used for concentration of DNA circulating in blood. Precipita...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytical biochemistry 2011-01, Vol.408 (2), p.354-356
Hauptverfasser:	Bryzgunova, Olga, Bondar, Anna, Morozkin, Evgeniy, Mileyko, Vladislav, Vlassov, Valentin, Laktionov, Pavel
Format:	Artikel
Sprache:	eng
Schlagworte:	acetone Acetone - chemistry blood DNA DNA probes DNA, Circular - blood DNA, Circular - chemistry DNA, Circular - isolation & purification ethanol Ethylamines - chemistry Fractional Precipitation - methods Humans Male microarray technology polymerase chain reaction Prostatic Neoplasms - blood Prostatic Neoplasms - genetics reliability reproducibility Sodium Acetate - chemistry triethylamine
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Concentration of circulating DNA probes is required to increase the amount of DNA involved in subsequent study (by polymerase chain reaction, sequencing, and microarray). This work was dedicated to the comparison of five different methods used for concentration of DNA circulating in blood. Precipitation of circulating DNA with acetone in the presence of triethylamine provides minimal DNA loss, high reproducibility, and at least three times higher DNA yield in comparison with the standard ethanol protocol.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/j.ab.2010.09.005